Topic:

Infection Control, Nosocomial Infections and Critical Care

Abstract No.:

ISE.006

Title:

Emergence of multi drug resistant bacillus species isolated from inanimate and animate hospital surfaces

Author(s):

S. Jalalpour; Lecture of Microbiology, Islamic Azad University Shahreza Branch, Lecture of Microbiology, Islamic Azad University Shahreza Branch, Esfahan/IR

Abstract:

Background: Hospital surfaces can serve as reservoirs of potential pathogen bacteria.Staff hands are more sources to transmission bacteria in hospital. A current phenomenon of great concern in the medical community in developing countries is raising multi-drug resistant organisms. Prevalence beta lactamase enzyme in staff hands bacteria and hospital surfaces bacteria, due to spread β–lactamase in bacteria and increase antibiotic resistance nosocomial infection. Subject of this study were survey and comparative frequency of beta lactamase enzyme and Antibiogram pattern in isolated Bacillus spp. from staff hand hospital surfaces in Iran.
Methods: The research was performed with laboratory method during 2005-2007 years in Azzahra hospital in Isfahan. Overall was study 194 strain from surface and 80 strain from staff hand .Environmental samples collected, with swab in Nutrient Broth (NB)and staff hand samples collected with Finger Print method, Bacterial identification were performed with Bacteriological methods, beta lactamase production with Acidometric method and Antibiogram pattern was performed with Kirby Bauer method.All the statistical analyses carried out using SPSS version 14. Chi-square and fisher test used for determination of significance of association. The p≤ 0.05 was considered significant.
Results: From 194 Bacterial strain isolated from hospital surface, Bacillus spp. 47(24%) and from 80 strain isolated from staff hands, Bacillus spp. 48(60%). According to Acidometric test result 96.15% of B.cereus strain and 36.165% of other Bacillus spp. produce β-lactamase.
Conclusion: Result shows high frequency of antibiotic resistant and beta lactamase producer Bacillus spp. on staff hands and hospital surfaces, high population bacteria in staff hands and hospital surfaces due to facility transmission Beta lactamase plasmid genes in bacteria. Regular monitoring antimicrobial drug resistance in the different areas is necessary to prevent unsuitable utilization of drugs which is the most important cause of emerging multi drug resistant strains. Establish systems for monitoring antimicrobial resistance in hospitals and the community and link these findings to resistance and disease surveillance data is fundamental to developing treatment guidelines accurately and to assessing the effectiveness of interventions appropriately.