Topic:

Diagnostics

Abstract No.:

ISE.043

Title:

Successful rarely using a sensitive PCR assay in adenovirus antigen diagnostic in children

Author(s):

A. Bajraktarevic¹, M. Miokovic², A. Mahinic³, S. Putica³, T. Frankic⁴, E. Hadzimuratovic Hadzipasic⁵, A. Hadzimuratovic⁵, M. Mujic⁵, D. Abduzaimovic⁶, A. Drnda⁷, A. Hadzimuratovic Senior⁸; ¹Public Health Institution of Canton Sarajevo, Pediatrics Department, Sarajevo/BA, ²Public Health Institution of Canton Sarajevo, Pediatrics department, Sarajevo/BA, ³Public Health Institution of Canton Sarajevo, Pediatrics Department, Sarajevo/BA, ⁴Pharmaceutical faculty Sarajevo , Department for Clinical Pharmacology, Sarajevo/BA, ⁵Pediatrics Clinic Sarajevo, Infectious Department, Sarajevo/BA, ⁶Public Health Institution of Canton Zenica, Department for Biochemistry, Tesanj/BA, ⁷Infectious Clinic Sarajevo , Pediatrics department, Sarajevo/BA, ⁸Surgery Clinic Sarajevo, Pediatrics Department, Sarajevo/BA

Abstract:

Background: Seroreactivity to adenovirus is common. By age 4 years, approximately half of all children have positive adenovirus titers. As a result, serology is less useful in the acute clinical setting. Adenoviruses are non-enveloped viruses with a linear double stranded DNA genome. Adenovirus is isolated most commonly in infants and children. Manifestations include symptoms of the common cold, pharyngitis, tonsillitis, otitis media, and pharyngoconjunctival fever.Aims: This study reports the design and validation of a sensitive and specific multiplex real-time PCR for the detection of a broad range of hAdV serotypes in respiratory samples in children.
Methods: Polymerase chain reaction PCR was being used with high specificity on various specimens as urine or blood) to identify adenovirus in children. Using polymerase chain reaction and antigen detection, a viral agent was identified in as many as 66% of the specimens. Serotyping was performed in eleven kids patients.
Results: Adenoviruses have a predilection for the respiratory tract and account for up to 9% of respiratory infections in younger children and an estimated about 6% of all infections in infants in Bosnian comparative groups of investigations. Viruses were documented in 44% of children. Notably, 22% of the children had concurrent acute viral and bacterial disease. The majority of febrile children in this study (51%) did not have an identified etiology for their fever.Discussion: There are 55 described serotypes in humans, which are responsible for 5–10% of upper respiratory infections in children, and many infections in adults as well.
Conclusion: The current methods of diagnosing respiratory viruses are limited in scope and sensitivity. Polymerase chain reaction is a more sensitive method than antigen detection and is often used for newly discovered viruses. In conclusion, the real time PCR-based antiviral assay is rapid, reproducible and could replace classical and more labor-intensive infectivity assays. Adenovirus antigens can be detected in less than half hour in a variety of body fluids from infected people by commercial immunoassay techniques.