Topic:

Bacterial infections

Abstract No.:

ISE.113

Title:

Bacteraemia caused by genus Paracoccus

Author(s):

V. Hasseltvedt¹, D. Caugant²; ¹Sykehuset Innlandet Trust, Dept. of Medical Microbiology, Lillehammer/NO, ²Norwegian Public Health Institute , Oslo/NO

Abstract:

Background: Genus Paracoccus consists of Gram-negative rod-shaped bacteria which may be found mostly in environmental samples. Paracoccus may also be engaged in the development of biofilm. In MEDLINE as of August 25, 2011, there was only one "hit" when it comes to peer-review articles on "Paracoccus" and "blood culture".We describe herein what may be the first documented Norwegian case of bacteraemia caused by Genus Paracoccus.
Methods: Blood cultures from a man, born 1928, admitted to our hospital in June 2011, were subjected primary tests at our laboratory, using standard media, i.e. blood, chocolate and lactose agars – incubated under aerobic and anaerobic conditions.
Results: Blood cultures werecultivation-positive for a Gram-negative,rod-shaped bacterium later identified belonging to Genus Paracoccus at the NIPH, which performed the reference testing, utilizing both traditional methods and 16S rRNA sequencing.Features:Sequencing:A 290 base pair fragment from the16S rRNA gene was sequenced, showing a 99% homology with the 16S RNA gene of Paracoccus sp. YT0095; indicating that the bacterium belongs to genus Paracoccus.Hemolysis: -Corrosion: -Growth on agars:Blood aerobic: 3+Chocolate aerobic: 3+Lactose aerobic: -Blood anaerobic: -Chocolate anaerobic: -Mannitol: -Nitrate reduction: -Catalase: +Oxidase: +OF Glucose: NegativeMotility at 30 degrees C: -Vancomycin 5 ug: Sensitive
Conclusion: In Norway bacteraemia caused by genus Paracoccus has not, to our knowledge, been described before. This is most likely be the first case case report ever in Norway demonstrating genus Paracoccus as the cause of bacteraemia/septicaemia. Other rather unusual findings from blood culture and "normally sterile sites" comprise, Granulicatella adjacens, Actinobaculum schalii, Actinobaculum urinale, Globicatella sulphidifaciens, Salmonella Farmingdale, Dialister sporosintes, Actinomyces neuii subspecies neuii, Anaerobiospirillum succiniciproducens, Leptotrichia trevisianii, Clostridium scindens, Genus Rahnella, Weeksella virosa, Bulleidea moorei and Haemophilus segnis.The example mentioned above shows the synergy that may be obtained between medical microbial laboratories on a regional and a central level.