Topic:

Zoonoses and Infections in Animals

Abstract No.:

ISE.122

Title:

Identification of Sarcocystis hominis in cattle in shahrekord district by PCR-RFLP method

Author(s):

H. Azizi, A. Borjian Boroujeni, M. Jafari; sharekord university, pathobiology, Sharekord/IR

Abstract:

Background: The parasites of genus Sarcocystis are intracellular protozoa parasites characterized by life cycles requiring 2 hosts. Herbivores (prey) and carnivores (predator) serve as the intermediate and definitive host, respectively. Three species of Sarcocystis have been recorded from cattle: S.cruzi, S.hirsuta and S.hominis with canids, felids, and primates, as definitive hosts, respectively. S.hominis has resulted in humans acquiring intestinal Sarcocystosis consist of nausea, stomachache and diarrhea.There are high infection rate of Sarcocystis in cattle in Iran but no study about Sarcocystis hominis identification.
Methods: In this study, heart, esophagus, diaphragm and tongue of 70 cattle were collected from different slaughterhouses of shahrekord district.DNA was extracted from templates by cetyl trimethyl ammonium bromide (CTAB) procedure adapted for small organisms. We used 18S9L and 18S1H for primers. Partial sequences of 18S rDNA were amplified with the forward primer 18S9L (5′-GGA TAA CCT GGT AAT TCT ATG-3′) and the reverse primer 18S1H (5′-GGC AAATGC TTT CGC AGT AG-3′). The amplified PCR products were digested separately with three restriction enzymes (Dra1, Fok1 and Bsl1) (Fermentas, USA). The obtained restriction fragments were separated on 2% agarose gels stained with Ethidium bromide (TBE-buffer, 55 V, 45 min).
Results: Amplification products were detected in 68.5% of samples which were further analyzed by RFLP. Results showed that the prevalence of S.hominis was 25.71%.