Topic:

Diagnostics

Abstract No.:

ISE.178

Title:

High antibody reactivity of three synthetics peptides with leprosy sera

Author(s):

L. Baassii¹, K. Sadki², F. Seghrouchni¹, R. El Aouad¹; ¹National Institute of hygiene 27, Av. Ibn Batouta, B.P. : 769 Rabat Morocco, Rabat/MA, ²Faculty of Science, University of Mohamed V, Rabat/MA

Abstract:

Background: The objective of this study was to evaluate the cross-reaction of six specific B-cell epitope peptides with sera from leprosy patients. These peptides were initially selected by in-silico method and synthesized for serodiagnosis of tuberculosis. 
Methods: In this study, twenty sera from leprosy patients, twenty sera from pulmonary tuberculosis and ten sera from healthy subjects were tested using an indirect ELISA peptide based method. 
Results: Among these six synthetic peptides, three showed high antibody reactivity with leprosy. Surprisingly, antibody responses against these three peptides were higher with leprosy sera than tuberculosis sera. 
Conclusion: These three peptides can be used in immunochromatographic test for the early diagnosis of leprosy.