Topic:

Antibiotic resistance

Abstract No.:

ISE.246

Title:

Study of selective antibiotic susceptibility against nasal infection

Author(s):

S. Bagde¹, R. K. Patidar², V. Singh³; ¹BARKATULLAH UNIVERSITY, Department of Microbiology, B.U Bhopal, Bhopal, MP/IN, ²BARKATULLAH UNIVERSITY, MICROBIOLOGY, Bhopal, MP/IN, ³Barkatullah University, Microbiology, Bhopal, M.P./IN

Abstract:

Background: Nasal infection or sinusitis is an inflammation of nasal passages caused by both viral and bacteriological pathogens. Antimicrobial resistance has universally recognized as growing problem concern about suitable therapy for nasal infection. The aim of study was to investigate the antibiotic susceptibility of some antibiotics against nasal infection causing agents.
Methods: Fifty clinical samples were taken from OPD of People's dental academy Bhopal and GMC Hospital, Bhopal (MP), India. Among fifty clinical samples, forty-seven bacterial strains were isolated out of which twenty-nine strains were of Gram positive bacteria (eight strains were of Staphylococcus aureus, six of Staphylococcus epidermidis, seven of Streptococcus pneumonia and eight of Corynebacterium diphtheria) and eighteen strains were of Gram negative bacteria (eight of Escherichia coli, six of Pseudomonas aeruginosa and four of Neisseria meningitides). Antimicrobial susceptibility assay was performed by disc diffusion method and  the reference criteria of clinical and laboratory standard institute guidelines.
Results: In the present study antibiotic susceptibility pattern results showed maximum level of resistance in gram positive strains S. aureus 8 (100%), S. epidermidis 6 (100%) and C. diphtheriae 8 (100%) against penicillin, S. aureus 8 (100%), S. epidermidis 6 (100%) and S. pneumoniae 7 (100%) were resistant to Cefuroxime,  S. aureus 7 (87.5%), S. epidermidis 6 (100%), S. pneumoniae 7 (100%) and C. diphtheriae 8 (100%) were resistant to Erythromycin and Azithromycin whereas, rest of gram positive strains showed satisfactory antibiotic susceptibility  against chloramphenical, tetracyclin,  cephalexin, cefazolin, ciprofloxacin and ofloxacin. Similarly for gram negative strains multi-drug resistance was observed in 8 (100%) isolates of E. coli against aztreonam, cefotaxime, ceftriaxone, nalidixic acid, cefuroxime, ciprofloxacin, ofloxacin, ceftazidime, cefixime and cefdinir, P. aeruginosa 6 (100%) were resistant to aztreonam, cefotaxime, ceftriaxone, nalidixic acid, cefuroxime, ofloxacin, ceftazidime, cefixime and cefdinir, N. meningitidis 4 (100%) were resistant to aztreonam, cefotaxime, nalidixic acid, ofloxacin, ceftazidime, cefixime and cefdinir whereas rest of gram negative strains showed moderate susceptible against ciprofloxacin, amikacin and cefuroxime.
Conclusion: Hence we concluded that the efficacy of cefazolin, cephalexin, chloramphenical, tetracyclin and ciprofloxacin was recorded higher than other antibiotics tested against nasal infection causing pathogens.