Topic:

Virology and viral infections (non-hiV)

Abstract No.:

ISE.258

Title:

Antigenic characterization of the pandemic Influenza H1N1pdm09 patients in Indonesia in 2009

Author(s):

N. K. Susilarini, V. Setiawaty, M. Mursinah, H. Hariandja, N. I. Hariastuti, K. N. Andriana Pangesti; Center for Biomedical and Basic Health Technology, National Institute of Health Research and Development, MOH, Jakarta/ID

Abstract:

Background: Until December 2009, in Indonesia, 1892 suspected cases of H1N1 were reported and all the laboratory examination were conducted at the Center for Biomedical and Basic Health Technology, National Institute of Health Research and Development (NIHRD). 890 positive cases were confirmed Influenza A/H1N1pdm09  and 12 cases ended in fatality (Unpublished data). Influenza is a disease caused by influenza viruses that normally infect human respiratory system that often require hospitalization until death. Antigenic characterization of influenza viruses were identified from the isolates which were obtained from clinical specimens. This step is an essential part of influenza surveillance in the whole world. In this study we did  conduct the antigenic characterization of influenza virus isolates from Influenza A/H1N1pdm09 cases in 2009 in Indonesia which have not been elucidated.
Methods: Positive clinical specimens Influenza A/H1N1pdm09 by RT-PCR from archive samples were cultured using MDCK cell. Grown cultures were confirmed by Hemagglutinasi (HA) test and then characterized using Hemagglutinasi inhibition (HI) methods with reference antisera A/California/07/2009 (H1N1) pdm and A / Influenza / Brisbane / H1N1pdm. 
Results: There are 205 specimens that met inclusion criteria for virus culture. All isolates that can be grown were detected by using Hemagglutinasi (HA) test. Total of 68 specimens or 33.2% out of 205 specimens were successfully grown and 3 specimens (1.46%) eventually were excluded because of contamination.Of the 68 positive isolates of influenza followed by Hemagglutinasi Inhibition (HI) examination to identify antigenic characterization using two reference antisera ; A/California/07/2009 (H1N1) and A/Influenza/Brisbane/H1N1pdm. All isolates (100%) showed antibodies to the antigen binding antisera A/California/07/2009 (H1N1) pdm.
Conclusion: All H1N1pdm09 viruses which were circulating in Indonesia in 2009 had an A/California/07/2009 (H1N1) pdm type.