Topic:

Zoonoses and Infections in Animals

Abstract No.:

ISE.307

Title:

Detection of brucellosis among patients admitted to Karaj Hospitals, Iran

Author(s):

F. Ezzatifar, A. Ebrahimzadeh Namvar, B. Asghari, A. Rastegar Lari*; Antimicrobial-Resistance Research Center , Department of Microbiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran/IR

Abstract:

Background: Brucellosis is a zoonotic disease that can be transmitted by direct or indirect contact with infected animal or their products.
Methods: This study was designed to detection of the presence of Brucella antibodies using commercial Brucella IgG and IgM ELISA kits. A total of 194 sera from suspected patients were received from 2 hospitals in Karaj from second part of 2011.
Results: Only 12 serum samples (6.1%) were positive for Brucella antibodies in which 6 demonstrated the presence of both IgM and IgG. Most of the positive patients were occupationally assocaited with animals.
Conclusion: This study recommends ELISA test detecting IgM and IgG antibody titers together found to be effective methods increasing the chance of brucellosis diagnosis, and could be helpful for follow up of brucellosis. The ELISA method described is simple and generally much easier to perform for a large number of serums and possibly the most reliable, consistent test for use in any eradication program for human brucellosis in Iran.