Topic:

Sexually transmitted diseases

Abstract No.:

ISE.378

Title:

Low and high risk human papillomavirus (HPV) in the oral mucosa of mexican women with genital human papillomavirus

Author(s):

A. M. Fernandez Presas¹, M. E. Manjarrez², D. Rosete³, S. Pedraza⁴, F. Mejia⁵, J. Delgado⁶; ¹Universidad Nacional Autonoma de Mexico, Microbiologia y Parasitologia, Mexico Distrito Federal, MEXICO DISTRITO FEDERAL/MX, ²Instituto Nacional de Enfermedades Respiratorias, Investigación en Virología y Micología, Mexico, D. F/MX, ³Instituto Nacional de Enfermedades Respiratorias Ismael Cosio Villegas, Investigación en Virología y Micología, Mexico/MX, ⁴Hospital Español., Gynecology, Mexico Distrito Federal/MX, ⁵Instituto Nacional de enfermedades Respiratorias Ismael Cosio Villegas, Investigación en Virología y Micología, Mexico/MX, ⁶Universidad Nacional Autónoma de México, México, DF/MX

Abstract:

Background: Human papillomaviruses are DNA viruses that infect the epithelium of the skin and mucous membranes. They are classified in low and high risk, based on their oncogenic potential. High risk HPV are implicated in cervical cancer and, nowadays, their role in oral cancer is being assessed, as well as the existing relation between the two sites of infection. In Mexico, there are few studies on oral cancer, therefore the interest in identifying the HPV frequency of low and high risk in samples of the oral and cervical cavities, as well as in determining some risk factors. The purpose on this pilot study was to determine the frequency of high and low risk HPV infection human papillomavirus in the oral cavity of women with cervical (HPV), and to correlate the infection site and its possible risk factors.
Methods: Eighteen female patients between 24 and 53 years, with antecedents of genital HPV infection were analyzed. DNA extraction from the epithelial cells was performed using the Qiagen kit. PCR was performed and the amplicon was observed in 2% agarose gels stained with ethidium bromide. Positive and negative controls were included in the reactions.
Results: HPV-DNA was detected in the 67% of both samples analyzed; cervix and oral cavity. The frequency of oral and cervix low risk HPV-DNA was 50%) in both samples, while high risk HPV-DNA in oral cavity was detected in 17%, and 39% in the cervix. The 100% of oldest group age participants (42-53) was infected with HPV. The study of the risk factors involved in HPV infection showed that the participants had the habits of smoking 39%; alcohol drinking 28%; and 78% oral sex.
Conclusion: The results of the present investigation showed a high frequency of HPV (67%) infection in the oral and genital mucosas, suggesting that patient’s habits could contribute to the infection; however, the smaller sample size don´t let us to reach a conclusion whether the HPV transmission goes from the oral cavity to the genital region or vice versa.