Topic:

Diagnostics

Abstract No.:

ISE.399

Title:

Investigation of Herpes Simplex Virus DNA in cerebrospinal fluid of suspected patients

Author(s):

M. Ziyaeyan, S. Assaei; Shiraz University of Medical Sciences, Clinical Microbiology Research Center, Shiraz/IR

Abstract:

Background: Herpes simplex virus meningoencephalitisis an acute infection accompanied by significant morbidity and mortality. PCR has become the gold standard for the diagnosis of HSV infection in the central nervous system. Our objective was to use of a real-time PCR assay that reliably and accurately detects HSV in Cerebrospinal fluid (CSF).
Methods: CSF samples from 167 patients having typical symptoms of HSV CNS infection collected in sterile containers were transported immediately to the laboratory for processing. The samples were extracted by a standard spin column extraction method and analysed by real-time PCR targeting a type common region of HSV1 and HSV2.
Results: Of 167 samples tested by real-time PCR, virus was detected in 14 patients (8%).Eight PCR positive patients were male and 6 were female.
Conclusion: Real-time PCR provides a fast and specific diagnosis. It was also considered as an early, rapid and sensitive diagnostic test andenhances specific diagnosis of CNS herpetic infection and allows timely, effective treatment leading to better outcomes.