Topic:

Antibiotic resistance

Abstract No.:

ISE.429

Title:

The resistance to antibiotics of Staphylococcus aureus strains isolated from hospitalized patients in Universities Hospitals from Cluj Napoca, Romania and in Saint George General Hospital of Chania, Greece

Author(s):

L. M. Junie¹, L. M. Simon¹, S.-L. Pandrea¹, S. Kastanakis², B. Tsagli³, L. Kalogeraki⁴, M. Papadogianni⁴, E. Vardaki³, I. Lagoudaki², G. Aleuraki³; ¹University of Medicine and Pharmacy , Microbiology, Cluj Napoca/RO, ²Saint George General Hospital, 1st Medical Department, Chania/GR, ³St. George General Hospital Chania, Microbiology, Chania/GR, ⁴Saint George general hospital, Comity for Infectious Control , Chania/GR

Abstract:

Background: Staphylococcus aureus infections remain a serious medical problem and prompt initiation of appropriate antimicrobial chemotherapy is important to improve the prognosis of these infections. The aim of the study was to identify the resistant phenotypes among circulating Staphylococcus strains in Romania and Greece, during 2008 and 2010. We studied in comparison the prevalence and susceptibility to antimicrobial agents of Staphylococcus aureus isolates from hospitalized patients with bloodstream and others infections.
Methods: All cultures were incubated into the BacT/alert (Biomérieux) blood culture system. Species identification was achieved by standard procedures and by the automated system Vitek 2 (Biomérieux). The susceptibility tests were performed using the disk diffusion method (Kirby-Bauer) according to the CLSI criteria and by Vitek 2 System, for a total of 1268 strains in Greece and in Romania).
Results: At Hospital of Chania, from 6494 blood cultures sets, positive cultures were 855 (13,2) and 51 of them (5,96) yielded Staphylococcus aureus. 48,03% of S. aureus isolated strains were MRSA in Romania and 27,5% in Greece (55,6% in 2009 and 12,1% in 2010). Fig.1 All results are listed in the attached tables. S. aureus tested strains were resistant to clindamycin (33,3%/34,06% and 6,1% in 2010), to gentamicin (72,92% in Ro, 23,5% in Gr). Moderately increased percentages of resistance were found by testing the strains to trimethoprim/sulfamethoxazole (13,97%) in Ro. Low levels of resistance were observed by testing the strains to ciprofloxacin in Ro (9,61%/5,5%). All the S. aureus strains tested to glycopeptide antibiotics and the second line antistaphylococcal antibiotics: linezolid, quinupristin/dalfopristin was found sensitive. All the S. aureus strains tested in Gr were sensitive to all antibiotics in 2010.